Compositions comprising salbutamol sulphate

ABSTRACT

A pharmaceutical composition is described that is suitable for delivery from a pressurised container. The composition is free of polar excipients and comprises: (a) a propellant component that consists essentially of 1,1-difluoroethane (R-152a); (b) a surfactant component that comprises oleic acid; and (c) a drug component that consists of salbutamol sulphate. The pharmaceutical composition can be delivered using a metered dose inhaler (MDI).

The present invention relates to a pharmaceutical composition comprising salbutamol sulphate, a propellant and a surfactant. The composition is suitable for delivering the salbutamol sulphate from a pressurised aerosol container using a metered dose inhaler (MDI).

MDIs are the most significant type of inhalation drug delivery system and are well known to those skilled in the art. They are designed to deliver, on demand, a discrete and accurate amount of a drug to the respiratory tract of a patient using a liquefied propellant in which the drug is dissolved, suspended or dispersed. The design and operation of MDIs is described in many standard textbooks and in the patent literature. They all comprise a pressurised container that holds the drug formulation, a nozzle and a valve assembly that is capable of dispensing a controlled quantity of the drug through the nozzle when it is activated. All of these components are typically located in a housing that is equipped with a mouth piece. The drug formulation will comprise a propellant, in which the drug is dissolved, suspended or dispersed, and may contain other materials such as polar excipients, surfactants and preservatives.

In order for a propellant to function satisfactorily in MDIs, it needs to have a number of properties. These include an appropriate boiling point and vapour pressure so that it can be liquefied in a closed container at room temperature but develop a high enough pressure when the MDI is activated to deliver the drug as an atomised formulation even at low ambient temperatures. Further, the propellant should be of low acute and chronic toxicity and have a high cardiac sensitisation threshold. It should have a high degree of chemical stability in contact with the drug, the container and the metallic and non-metallic components of the MDI device, and have a low propensity to extract low molecular weight substances from any elastomeric materials in the MDI device. The propellant should also be capable of maintaining the drug in a homogeneous solution, in a stable suspension or in a stable dispersion for a sufficient time to permit reproducible delivery of the drug in use. When the drug is in suspension in the propellant, the density of the liquid propellant is desirably similar to that of the solid drug in order to avoid rapid sinking or floating of the drug particles in the liquid. Finally, the propellant should not present a significant flammability risk to the patient in use. In particular, it should form a non-flammable or low flammability mixture when mixed with air in the respiratory tract.

Dichlorodifluoromethane (R-12) possesses a suitable combination of properties and was for many years the most widely used MDI propellant, often blended with trichlorofluoromethane (R-11). Due to international concern that fully and partially halogenated chlorofluorocarbons (CFCs), such as dichlorodifluoromethane and trichlorofluoromethane, were damaging the earth's protective ozone layer, many countries entered into an agreement, the Montreal Protocol, stipulating that their manufacture and use should be severely restricted and eventually phased out completely. Dichlorodifluoromethane and trichlorofluoromethane were phased out for refrigeration use in the 1990's, but are still used in small quantities in the MDI sector as a result of an essential use exemption in the Montreal Protocol.

1,1,1,2-tetrafluoroethane (R-134a) was introduced as a replacement refrigerant and MDI propellant for R-12. 1,1,1,2,3,3,3-heptafluoropropane (R-227ea) was also introduced as a replacement for dichlorotetrafluoroethane (R-114) in the MDI sector and is sometimes blended with R-134a for this application.

Although R-134a and R-227ea have low ozone depletion potentials (ODPs), they have global warming potentials (GWPs), 1430 and 3220 respectively, that are now considered to be too high by some regulatory bodies, especially for dispersive uses when they are released into the atmosphere.

One industrial area that has received particular attention recently has been the automotive air-conditioning sector where the use of R-134a has come under regulatory control as a result of the European F-Gas Regulations. Industry is developing a number of possible alternatives to R-134a in automotive air conditioning and other applications that have a low greenhouse warming potential (GWP) as well as a low ozone depletion potential (ODP). Many of these alternatives include hydrofluoropropenes, especially the tetrafluoropropenes, such as 2,3,3,3-tetrafluoropropene (R-1234yf) and 1,3,3,3-tetrafluoropropene (R-1234ze).

Although the proposed alternatives to R-134a have a low GWP, the toxicological status of many of the components, such as certain of the fluoropropenes, is unclear and they are unlikely to be acceptable for use in the MDI sector for many years, if at all.

There are also other problems with R-134a and R-227ea. Most pharmaceutical actives for treating respiratory disorders, such as asthma, tend not to dissolve well in either R-134a or R-227ea and have to be handled as suspensions in the propellant. Drug suspensions give rise to a number of problems, such as nozzle blockage, agglomeration and sedimentation, the latter problem making it essential to shake the MDI thoroughly before use to ensure that the drug is evenly distributed in the propellant. Furthermore, if the pharmaceutical active settles quickly following re-suspension in the propellant, as is often the case, then the propellant/drug composition must be delivered from the MDI shortly after shaking in order to ensure that the dose that is delivered contains an effective concentration of the pharmaceutical active.

The problem of poorly dissolving drugs has been addressed by including a polar excipient in the composition which either helps to dissolve the drug to form a solution or else enhances wetting of suspended drug particles to yield a better dispersed and more stable suspension. A preferred polar excipient is ethanol. However, the use of large amounts of ethanol can tend to result in a coarse spray having droplet sizes that are too large for acceptable penetration into the deep bronchiole passages of the lung. Further, high levels of ethanol can have unacceptable irritancy to the mouth and throat, especially with younger users. Clearly it would be advantageous to reduce the amount of ethanol that is required to produce an acceptable formulation. It would be better still if the use of ethanol could be avoided altogether.

Surfactants have also been included in some formulations that include drugs that are either insoluble or only sparingly soluble in the propellant, as these can also help to produce a more stable suspension. Unfortunately, many of the toxicologically acceptable surfactants have sufficient solubility in either R-134a or R-227ea. As a result, ethanol has been added to the composition, where it functions not only as a wetter but also as a solvent for the surfactant. It would be beneficial to find a propellant/surfactant combination that allows for sufficient surfactant to be dissolved in the propellant without the inclusion of a polar excipient such as ethanol or with reduced levels of such an excipient.

There is a need for a MDI aerosol formulation that has a reduced GWP in comparison with R-134a and R-227ea, that has acceptable flammability and toxicity performance, which forms stable suspensions and that has reduced irritancy.

According to a first aspect of the present invention there is provided a pharmaceutical composition that is free of polar excipients, said composition comprising:

-   -   a propellant component consisting essentially of         1,1-difluoroethane (R-152a),     -   a surfactant component comprising oleic acid; and     -   a drug component consisting of salbutamol sulphate.

According to a second aspect of the present invention there is provided a pharmaceutical composition consisting essentially of:

-   -   a propellant component consisting essentially of         1,1-difluoroethane (R-152a),     -   a surfactant component comprising oleic acid; and     -   a drug component consisting of salbutamol sulphate.

The pharmaceutical compositions of the first and second aspects of the present invention are suitable for delivery from a pressured container, e.g. using a metered dose inhaler (MDI).

The pharmaceutical compositions of the first and second aspects of the present invention typically comprise from 0.01 to 1.0 weight % of the drug component, from 96.5 to 99.94 weight % of the propellant component and from 0.05 to 2.5 weight % of the surfactant component. Preferred compositions comprise from 0.05 to 0.5 weight % of the drug component, from 97.5 to 99.85 weight % of the propellant component and from 0.1 to 2.0 weight % of the surfactant component. Particularly preferred pharmaceutical compositions comprise from 0.07 to 0.2 weight % of the drug component, from 98.8 to 99.73 weight % of the propellant component and from 0.2 to 1.0 weight % of the surfactant component. All percentages are based on the total weight of the pharmaceutical compositions.

The propellant component in the pharmaceutical compositions of the first and second aspects of the present invention consists essentially of 1,1-difluoroethane (R-152a). Thus, we do not exclude the possibility that the propellant component may include small amounts of propellant compounds in addition to the R-152a. For example, the propellant component may additionally comprise one or more additional hydrofluorocarbon or hydrocarbon propellant compounds, e.g. selected from R-227ea, R-134a, difluoromethane (R-32), propane, butane, isobutane and dimethyl ether. If an additional propellant compound is included, the R-152a will constitute at least 90 weight %, e.g. from 90 to 99 weight % of the propellant component. Preferably, the R-152a will constitute at least 95 weight %, e.g. from 95 to 99 weight %, and more preferably at least 99 weight % of the propellant component. In an especially preferred embodiment, the propellant component is entirely R-152a, so that the pharmaceutical compositions of the invention comprise R-152a as the sole propellant.

As well as the oleic acid, the surfactant component in the pharmaceutical compositions of the first and second aspects of the present invention may also comprise one or more additional surfactants to aid in the dispersion of solid drug particles in the propellant. Suitable additional surfactants include ethyl oleate, sorbitan trioleate, isopropyl myristate, polyethylene glycol 300, polyoxyethylene 20 sorbitan monooleate and monolaurate, and propoxylated polyethyleneglycol.

Preferably, the surfactant component consists entirely of oleic acid. Accordingly, in a preferred embodiment of the present invention, the percentages listed above for the typical and preferred amounts of the surfactant component in the pharmaceutical compositions of the present invention define the typical and preferred amounts of oleic acid in those compositions.

By the terms “consists of” and “consisting of” as used herein, we are intending to exclude the presence of additional components. Thus, the drug component in the pharmaceutical compositions of the present invention consists entirely of salbutamol sulphate, so that the only drug in the pharmaceutical compositions is salbutamol sulphate. The salbutamol sulphate does not dissolve or dissolve significantly in the propellant component but forms a dispersion or suspension in the propellant/surfactant mixture. The suspended drug particles preferably have a diameter of less than 100 microns.

The pharmaceutical composition of the first aspect of the present invention is free of polar excipients. Polar excipients, such as ethanol, are used routinely in pharmaceutical compositions for treating respiratory disorders that are delivered using metered dose inhalers (MDIs). They are also referred to as solvents, co-solvents, carrier solvents and adjuvants. Their inclusion can serve to solubilise the surfactant or the drug in the propellant and/or inhibit deposition of drug particles on the surfaces of the metered dose inhaler that are contacted by the pharmaceutical composition as it passes from the container in which it is stored to the nozzle outlet. They are also used as bulking agents in two-stage filling processes where the drug is mixed with a suitable polar excipient. The most commonly used polar excipient is ethanol.

The present inventors have discovered that for salbutamol sulphate, the use of R-152a as the propellant and oleic acid as the surfactant mitigates the need for polar excipients and allows compositions that are free of polar excipients, and especially ethanol, to be prepared that still deliver good performance when delivered from a medication delivery device, such as a metered dose inhaler (MDI).

The pharmaceutical composition of the first aspect of the present invention preferably consists essentially of and more preferably consists entirely of the three listed components. By the term “consists essentially of”, we mean that at least 95 weight %, more preferably at least 98 weight % and especially at least 99 weight % of the pharmaceutical composition consists of the three listed components.

The pharmaceutical composition of the second aspect of the present invention consists essentially of and preferably consists entirely of the three listed components. By the term “consists essentially of”, we mean that at least 95 weight %, more preferably at least 98 weight % and especially at least 99 weight % of the pharmaceutical composition consists of the three listed components.

Although not preferred, the pharmaceutical composition of the second aspect of the present invention optionally contains at least one polar excipient. In principal, any polar material that is pharmaceutically acceptable may be employed as a polar excipient. Examples of suitable polar excipients include alcohols, such as ethyl alcohol (ethanol) and glycerol, and glycols, such as propylene glycol, polyethylene glycols and polypropylene glycols. The most preferred polar excipient is ethanol, which may be used together with other polar excipients but is preferably used alone. Preferably, the pharmaceutical composition of the second aspect of the present invention is free of any polar excipients such as ethanol.

Where a polar excipient is employed, the mandatory and preferred amounts of R-152a in the propellant component are as discussed above. Preferably, the propellant component will consist entirely of R-152a and the surfactant component will consist entirely of oleic acid even when a polar excipient is present.

The pharmaceutical compositions of the first and second aspects of the present invention find particular utility in the delivery of salbutamol sulphate from a pressurised aerosol container using a metered dose inhaler (MDI). In this application, the propellant component functions to deliver the drug as a fine aerosol spray.

In an especially preferred embodiment, the present invention provides a pharmaceutical composition for delivery from a pressurized container that is free of polar excipients comprising:

-   -   a propellant component consisting of 1,1-difluoroethane         (R-152a);     -   a surfactant component consisting of oleic acid; and     -   a drug component consisting of salbutamol sulphate.

In this especially preferred embodiment, the pharmaceutical composition preferably consists essentially of and more preferably consists entirely of the three listed components.

The pharmaceutical compositions of the invention may also comprise one or more other additives of the type that are conventionally used in drug formulations for pressurised MDIs, such as valve lubricants. Where other additives are included in the pharmaceutical compositions, they are normally used in amounts that are conventional in the art.

The pharmaceutical compositions of the invention are normally stored in pressurised containers or canisters which are to be used in association with a medication delivery device. When so stored, the pharmaceutical compositions are normally in the liquid state. In a preferred embodiment, the pressurised container is designed for use in a metered dose inhaler (MDI).

Accordingly, a third aspect of the present invention provides pressurised containers holding respectively the pharmaceutical compositions of the first and second aspects of the present invention. In a fourth aspect, the present invention provides medication delivery devices, especially metered dose inhalers, having pressurised containers respectively holding the pharmaceutical compositions of the first and second aspects of the present invention.

In an especially preferred embodiment, the present invention provides a pressurised container holding a pharmaceutical composition that is free of polar excipients comprising:

-   -   a propellant component consisting of 1,1-difluoroethane         (R-152a);     -   a surfactant component consisting of oleic acid; and     -   a drug component consisting of salbutamol sulphate.

In another especially preferred embodiment, the present invention provides a medication delivery device, especially a metered dose inhaler, having a pressurised container holding a pharmaceutical composition that is free of polar excipients comprising:

-   -   a propellant component consisting of 1,1-difluoroethane         (R-152a);     -   a surfactant component consisting of oleic acid; and     -   a drug component consisting of salbutamol sulphate.

In these especially preferred embodiments, the pharmaceutical composition preferably consists essentially of and more preferably consists entirely of the three listed components.

The typical and preferred proportions of the drug component, propellant component and surfactant component in the pharmaceutical composition of these especially preferred embodiments are as discussed above.

The pharmaceutical compositions of the present invention are for use in medicine for treating a patient suffering or likely to suffer from a respiratory disorder and especially asthma.

Accordingly, the present invention also provides a method for treating a patient suffering or likely to suffer from a respiratory disorder, especially asthma, which comprises administering to the patient a therapeutically or prophylactically effective amount of a pharmaceutical composition as discussed above. The pharmaceutical composition is preferably delivered to the patient using a MDI.

The pharmaceutical compositions of the invention can be prepared by a simple blending operation in which the R-152a-containing propellant component, the oleic acid-containing surfactant component, and the salbutamol sulphate are mixed together in the required proportions in a suitable mixing vessel. Mixing can be promoted by stirring as is common in the art. Conveniently, the R-152a-containing propellant component is liquefied to aid mixing. If the pharmaceutical composition is made in a separate mixing vessel, it can then be transferred to pressurised containers for storage, such as pressurised containers that are used as part of medication delivery devices and especially MDIs.

The pharmaceutical compositions of the invention can also be prepared within the confines of a pressurised container, such as an aerosol canister or vial, from which the compositions are ultimately released as an aerosol spray using a medication delivery device, such as a MDI. In this method, a weighed amount of the salbutamol sulphate is introduced into the open container. A valve is then crimped onto the container and the 152a-containing propellant component, in liquid form, introduced through the valve into the container under pressure, optionally after first evacuating the container through the valve. The oleic acid-containing surfactant component can be mixed with the salbutamol sulphate or, alternatively, introduced into the container after the valve has been fitted, either alone or as a premix with the propellant component. The whole mixture can then be treated to disperse the drug in the propellant or propellant/surfactant mixture, e.g. by vigorous shaking or using an ultrasonic bath. Suitable canisters may be made of plastics, metal or glass.

The canister may be filled with enough of the pharmaceutical composition to provide for a plurality of dosages. The pressurized aerosol canisters that are used in MDIs, typically contain 50 to 150 individual dosages.

For pharmaceutical compositions that comprise a drug in suspension in a propellant, the problem can arise that the suspended drug particles deposit on the interior surfaces of the canister and the valve of the drug delivery device. This problem can necessitate providing the canister interior with a special lining or coating, such as a fluoropolymer coating, and making the valves from specialist polymer materials. However, the pharmaceutical compositions of the invention are capable of forming a stable dispersion of the drug, thereby avoiding the problem of drug deposition, and yet deliver the drug as a sufficiently fine aerosol mist that is able to deliver the drug deep into the lung.

The present invention is now illustrated but not limited by the following examples.

EXAMPLE 1

A number of experiments were conducted to investigate the in vitro aerosolization performance of salbutamol sulphate in metered dose inhalers (MDIs) containing either R-134a or R-152a. The particle size of the salbutamol sulphate was less than 5 μm making it suitable for inhaled drug delivery.

A formulation containing salbutamol sulphate, oleic acid and R-152a was prepared. The drug and oleic acid were weighed directly into standard aluminium 19 mL cans (C128, Presspart, Blackburn, UK). The can was crimped with a 50 μL valve and the R-152a was then filled into the cans through the valve using a manual Pamasol crimper/filler (Pamasol, Switzerland). The can was then sonicated for 90 mins to ensure dissolution of the surfactant in the propellant and dispersion of drug in the medium. The final concentration of oleic acid in the formulation was 0.05 w/w.

A comparative formulation containing salbutamol sulphate, ethanol, oleic acid and R-134a was prepared. Intense mixing and sonication for 60 mins was employed to dissolve the oleic acid in the ethanol. The drug was weighed directly into standard aluminium 19mL cans (C128, Presspart, Blackburn, UK), to which an appropriate amount of the oleic acid/ethanol solution was added such that the final concentrations of oleic acid and ethanol were 0.05 and 15% w/w respectively. This slurry was further sonicated for 60 mins in order to disperse the drug in the ethanol. The can was then crimped with a 50 μL valve (Bespak, Kings Lynn, UK). Finally, the R-134a was then filled into the cans through the valve using a manual Pamasol crimper/filler (Pamasol, Switzerland).

High performance liquid chromatography (HPLC) was used to determine drug content following aerosolization studies (see below). The HPLC machine consisted of a pump, column oven, column coupled to a UV detector (all Agilent 1200, Wokingham, Berkshire, UK). A Hypersil BDS C18 column (Fisher, Loughborough, UK, 5 μm, 250×4.6 mm i.d.) was used for high-throughput analysis of samples. The chromatographic conditions for the salbutamol sulphate are shown in Table 1.

TABLE 1 Pump Flow UV Column Rate Wavelength Temperature Drug (ml · min⁻¹) Mobile Phase (nm) (° C.) Salbutamol 1.8 Methanol:Water 240 60 Sulphate (0.25% W/V 1- (Sal SO₄) heptane sulfonic acid sodium salt) (40:60 V/V)

The in vitro aerosolization performance of the formulation was studied using a Next Generation Impactor (NGI, Copley Scientific, Nottingham UK), which was connected to a vacuum pump (GE Motors, N.J., USA). Prior to testing, the cups of the NGI system were coated with 1% v/v silicone oil in hexane to eliminate particle bounce. For each experiment, three actuations of the can were discharged into the NGI at 30 L.min⁻¹ as per pharmacopeia guidelines. Following aerosolization, the NGI apparatus was dismantled and the actuator and each part of the NGI was washed down into known volumes of the HPLC mobile phase. The mass of drug deposited on each part of the NGI was determined by HPLC. This protocol was repeated three times for the can, following which, the fine particle dose (FPD) and fine particle fraction of the emitted dose (FPF_(ED)) were determined.

The in vitro aerosolization performance of salbutamol sulphate following aerosolization from MDI formulations containing HFA134a, oleic acid and ethanol or HFA152a and oleic acid is summarised in Table 2 and shown in FIG. 1.

These data show that the emitted dose of salbutamol sulphate from both formulations was similar. However, the FPD and % FPF_(ED) for the formulation containing R-152a and oleic acid was significantly (p<0.05) greater than the formulation containing R-134a, oleic acid and ethanol. The stage-by-stage data represented in FIG. 1 shows that the R-152a/oleic acid formulation had significantly (p<0.05) less throat deposition, and significantly (p<0.05) greater deposition on stage 4.

TABLE 2 Emitted Fine Particle Dose Dose FPF_(ED) MMAD ± (μg ± S.D.) (μg ± S.D.) (%) GSD Salbutamol/Oleic acid/ 99.3 (2.8) 26.4 (1.5) 26.6 (0.8) 2.60 (1.80) ethanol/HFA134a Salbutamol/Oleic acid/ 98.4 (2.7) 30.9 (0.1) 30.4 (0.9) 3.10 (1.80) HFA152a MMAD = mass median aerodynamic diameter GSD = geometric standard deviation

EXAMPLE 2

A number of experiments were conducted to investigate the solubility of various surfactants in R-134a and R-152a. The apparent surfactant dissolution in each propellant was studied at ambient temperature (19.5-25.0 ° C.) by adding a known quantity of surfactant to a plastic-coated, pressure-resistant, clear glass aerosol bottle. A continuous flow valve was then crimped onto the bottle. A total of 10 grams of each propellant was filled into each aerosol bottle such that the surfactant concentration ranged from 0 to 5% w/w. After addition of propellant, the contents were sonicated for 10 mins and viewed with the naked eye for dissolution.

The results are shown in the tables below.

TABLE 3 Solubility of oleic acid in different propellants Solubility of oleic acid in the Propellant propellant (% w/w) R-134a Up to 0.05 R-152a Up to 2.5 

TABLE 4 Solubility of lecithin in different propellants Solubility of lecithin in the Propellant propellant (% w/w) R-134a Up to 0.01 R-152a Up to 0.05

TABLE 5 Solubility of polyvinylpyrrolidone in different propellants Solubility of polyvinylpyrrolidone Propellant in the propellant (% w/w) R-134a Up to 0.01 R-152a Up to 0.01

TABLE 6 Solubility of PEG 400 in different propellants Solubility of PEG 400 in the Propellant propellant (% w/w) R-134a Up to 0.02 R-152a Up to 0.05

TABLE 7 Solubility of sorbitan monooleate in different propellants Solubility of sorbitan monooleate in Propellant the propellant (% w/w) R-134a Up to 0.03 R-152a Up to 0.05

TABLE 8 Solubility of sorbitan trioleate in different propellants Solubility of sorbitan trioleate in the Propellant propellant (% w/w) R-134a Up to 0.05 R-152a Up to 0.05

It is apparent from the results shown above that the R-152a/oleic acid propellant/surfactant combination is particularly good, with oleic acid exhibiting much higher solubility in R-152a than R-134a and much higher solubility in R-152a than any other surfactant. 

1. A pharmaceutical composition consisting essentially of: (a) 1,1-difluoroethane (R-152a), (b) oleic acid; and (c) salbutamol sulphate. 2-6. (canceled)
 7. A sealed container that contains a pharmaceutical composition as claimed in claim
 1. 8. The sealed container of claim 7 which is a pressurized container for use with a metered dose inhaler (MDI).
 9. A metered dose inhaler (MDI) fitted with a pressurized container as claimed in claim
 8. 10. A method for treating a patient suffering or likely to suffer from a respiratory disorder which comprises administering to the patient a therapeutically or prophylactically effective amount of a pharmaceutical composition as claimed in claim
 1. 11. The method of claim 10, wherein the respiratory disorder is asthma.
 12. The method of claim 10, wherein the pharmaceutical composition is delivered to the patient using a metered dose inhaler (MDI).
 13. A method for manufacturing a pharmaceutical composition as claimed in claim 1, said method comprising the steps of: introducing a weighed amount of the salbutamol sulphate into an open container from which the salbutamol sulphate will ultimately be released as an aerosol spray using a medication delivery device; fitting a valve device onto the container; introducing the 1,1-difluoroethane, in liquid form, through the valve into the container under pressure; and introducing the oleic acid through the valve into the container under pressure.
 14. The method of claim 13, wherein the liquid 1,1-difluoroethane is mixed together with the oleic acid and the resulting liquid mixture introduced into the container under pressure via the valve.
 15. The pharmaceutical composition of claim 1, wherein the composition is free of polar excipients.
 16. The pharmaceutical composition of claim 1, wherein at least 99 weight % of the pharmaceutical composition consists of components (a), (b) and (c).
 17. The pharmaceutical composition of claim 15 which consists entirely of components (a), (b) and (c).
 18. The pharmaceutical composition of claim 17 which comprises from 0.01 to 1.0 weight % of salbutamol sulphate, from 96.5 to 99.94 weight % of 1,1-difluoroethane and from 0.05 to 2.5 weight % of oleic acid. 